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KMID : 0985420140360020092
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Laboratory Medicine and Quality Assurance
2014 Volume.36 No. 2 p.92 ~ p.98
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Evaluation of a Fully Automated, Rapid Detection System for CYP2C19 and UGT1A1 Genotyping
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Jeon Yong-Bum
Lee Seung-Jun
Cho Sung-Im
Seo Soo-Hyun
Ra Eun-Kyung
Park Seung-Man
Seong Moon-Woo
Park Sung-Sup
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Abstract
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Background : The need for genotyping single nucleotide polymorphisms (SNPs) in genes encoding drug-metabolizing enzymes is increasing. Therefore, the recent focus has been on developing fully automated methods for the rapid and accurate measurement of SNPs. Methods: We used the quenching probe (QP) method and i-densy IS-5310 to genotype 200 DNA specimens from 200 healthy Koreans and 100 whole blood from another 100 for the SNPs CYP2C19*2 and CYP2C19*3. We also performed genotyping of UGT1A1*6 and UGT1A1*28 with the above mentioned 200 DNA samples and 81 whole blood samples. The results of the assay were then compared to conventional direct sequencing.
Method : We used the quenching probe (QP) method and i-densy IS-5310 to genotype 200 DNA specimens from 200 healthy Koreans and 100 whole blood from another 100 for the SNPs CYP2C19*2 and CYP2C19*3. We also performed genotyping of UGT1A1*6 and UGT1A1*28 with the above mentioned 200 DNA samples and 81 whole blood samples. The results of the assay were then compared to conventional direct sequencing.
Result : The allele frequencies of CYP2C19 were 25.7% for *2 and 10.3% for *3, and those of UGT1A1 were 17.3% for *6 and 11.2% for *28. These results are similar to those reported in previous studies on Korean populations. The CYP2C19 and UGT1A1 genotypes determined by the QP method perfectly matched (100.0%, Κ=1.000, P<0.001 for CYP2C19, and 99.6%, Κ=0.992, P<0.001 for UGT1A1) those determined by direct sequencing, barring a single exception for the UGT1A1 genotype in 1 DNA specimen.
Conclusion : Our results suggest that the QP method, owing to its speed and ease of use, will enable rapid and sensitive diagnosis in clinical laboratories.
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KEYWORD
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Genotyping, CYP2C19, UGT1A1, Single nucleotide polymorphism
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